Imaging and tracking HIV viruses in human cervical mucus

MOLECULAR BIOLOGY OF HUMAN IMMUNODEFICIENCY VIRUSES: HIV-l AND HIV-2

Antimicrobial activity of human cervical mucus.

The antibacterial activity of human cervical mucus (CM) was examined on standardized microbial colonized agar plates (agar diffusion test). In parallel, the lysozyme content of CM was determined by means of a turbidimetric test system in aliquots of the same CM specimens. Suspensions of living lyophilized Micrococcus lysedeikticus were used as bacterial substrate. Testing was performed in a tot...

Globular structure of human ovulatory cervical mucus.

Human cervical mucus is a heterogeneous mixture of mucin glycoproteins whose relative concentration changes during the ovulatory phases, thereby producing different mucus aggregation structures that can periodically permit the transit of spermatozoa for fertilization. In preovulatory phase, mucus is arranged in compact fiber-like structures where sperm transit is hindered. Previously, through o...

Enumerating viruses in coral mucus.

The distribution of viruses inhabiting the coral mucus remains undetermined, as there is no suitable standardized procedure for their separation from this organic matrix, principally owing to its viscosity and autofluorescence. Seven protocols were tested, and the most efficient separations were obtained from a chemical treatment requiring potassium citrate.

Counting viruses in coral mucus

3 4 5 6 Amandine Leruste, Thierry Bouvier, Yvan Bettarel 7 8 9 10 UMR 5119 ECOSYM – Université Montpellier 2IRDIFREMER CNRS (France) 11 12 13 Running head : Mucus-associated viruses 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 *Corresponding author : [email protected] 42 43 IRD-UMR 5119 Ecologie des Systèmes Marins Côtiers (ECOSYM) 44 Université de Mont...